Sampling and analysis consist of three easy steps

  1. Concentration
  2. Reaction
  3. Measurement


Water samples are filtered through a Millipore 0.22 PES express closed filter unit where the bacteria are collected on the surface of the membrane. This makes our technology uniquely advantaged over others as filtering eliminates potential background interferents and the closed cell reduces sample handling.


The filter unit is saturated with a surplus of enzyme substrate.

The enzyme substrate reacts with a naturally occurring bacterial enzyme and a fluorescent compound is released. The reaction time is approximately 15 - 30 minutes depending on the application.

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The amount of fluorescence is measured using a handheld battery operated fluorometer. The more fluorescence produced the more bacteria is present in the sample.

The result is reported as a Bactiquant value (BQV).

BQV is defined as fluorescence generated in a 250 ml water sample in 30 minutes at 23oC (73.4oF). Although sample volume and reaction times can be modified to meet a specific application, the analytical results are always adjusted to the standard conditions.

Multiple Sampling Options

Water samples may be collected directly from the water source using an outlet adapter or collected with a sampling bottle or syringe. Sampling bottles and syringe can be attached to a manifold and the liquid filtered using vacuum. The manifold allows simultaneous filtration of 5 – 15 samples. Low-volume water samples can also be collected using a syringe and manually filtered through the Millex filter unit.

Recommended sampling volumes range from 10 – 1000 ml depending on the application.

Bactiquant-water Applications

  • Source water/raw water quality
  • Water Plant (Operations, process control)
  • Industrial Process Water (CIP, Food stuff production)
  • Distribution Systems (Finished water, WSP)
  • Verification HACCP
  • Sustainable Systems (Recycle and closed loops systems)
  • Technical water (Electronics, cooling towers, humidification)
  • Tracking and delineating pollution events
  • Effluents
  • Recreational water
  • Aquaculture
  • Water Biostability Assessment

Bactiquant Water applications

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