ENZYME ACTIVITY

The Mycometer technology is based on the finding that it is possible to identify enzyme activities that characterizes  certain taxonomic groups of organisms. In 1999, the Company launched the Mycometer®-test for measuring the fungal biomass on surfaces. This test is based on the finding that the level of an enzyme activity ( β-N-acetylhexosaminidase, NAHA) correlates with the level of ergosterol, a known indicator of fungal presence. Later investigations have shown that a broad range of randomly chosen fungi contain very high levels of this enzyme activity, which seems to be ubiquitous in fungi. Bactiquant®-water for quantifying bacteria in liquids was launched in 2007. This product is based on the targeting of a bacterial hydrolase activity  which correlates with bacterial biomass in a wide range of water samples. Today, Mycometer holds several patents and trademarks. The Company's technology has been verified by the United States Environmental Agency (US-EPA). 

PRINCIPLE

The enzymes are localized in the microorganisms. So when the sample is contacted with a solution containing an artificial enzyme substrate, the substrate is hydrolyzed by the target enzyme and a fluorophore is released.

MEASUREMENTS

The amount of fluorophore released by the microbial enzymes can then be measured in a small field fluorometer. 

 

ROBUSTNESS

What makes this technology particularly robust and reproducible is that the fluorophore is released directly into the reaction solution. This means that there is no need for extracting the fluorophore from the cells. This is a great advantage over many other technologies, as extraction procedures can be difficult to reproduce and add significantly to the variability of the test results.