BactiQuant® is based on the detection and quantification of an enzyme activity which belongs to a family of enzymes categorized as hydrolases.
The enzyme activity is present in bacteria. The activity is measured using a fluorogenic enzyme substrate, which upon cleavage by the bacterial enzyme, releases a fluorophore that fluoresces (emits light).
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Enzyme
substrate
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Flourescent
compound
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/M%C3%A5leprincip_(1).gif)
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/M%C3%A5leprincip_(2).gif)
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/M%C3%A5leprincip_(3).gif)
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Bacteria
enzyme
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The fluorescence is measured using a fluorometer.
The analysis can be performed on-site within an hour.
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